By Shayne Cox Gad; Christopher P Chengelis
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1989) Release of labeled arachidonic acid Acid release Cytotoxicity Cytotoxicity (MIT) Cytotoxicity Inflammation mediator release Increases in/3-hexosaminidase levels in the media Lipid metabolism inhibition Yes DeLeo et al. (1988) Yes Yes Yes No Lamont et al. (1989) Gales et al. (1989) Swisher et al. (1988) Babich et al. (1989) Boyce et al. (1988) No Lei et al. (1986) No DeLeo et al. (1987) Yes Reinhardt et al. (1987) Yes (surfactants) Prottey and Ferguson (1976) Cell detachment Growth inhibition Increased membrane permeability Inflammation mediator release Reinhardt et al.
1988). Statistics and Experimental Design for Toxicologists, 2nd ed. Telford Press, Caldwell, NJ. J. (1981). Scientific Considerations in Monitoring and Evaluating Toxicological Research. Hemisphere, New York. Larson, D. , King, T. , Nelson, R. , and Gad, S. C. (1995). Information sources: Building and maintaining data files. In Safety Assessment for Pharmaceuticals (S. C. ). Van Nostrand Reinhold, New York. Lu, F. C. (1985). Basic Toxicology, pp. 68-95. Hemisphere, New York. Page, N. P. (1986).
2. Wrong route. 3. Wrong vehicle or form of test material. 4. In studies in which several dose levels are studied, the worst thing that can happen is to have an effect at the lowest level tested (not telling you what dose in animals, much less in man, is safe). The next worst thing is to not have an effect at the highest dose tested (generally meaning that you will not know what the signs of toxicity are and it will invalidate a study in the eyes of many regulatory agencies). 5. Making leaps of faith.