Apoptosis Methods in Toxicology by Perpetua M. Muganda

By Perpetua M. Muganda

This unique quantity presents a unmarried, helpful reference resource for tactics that definitively establish and adequately quantify apoptosis. The publication starts off with universal tools applied to become aware of and quantitate apoptosis, in addition to apoptosis signaling pathways in toxicological and different similar learn. It keeps with multi-parametric and phased apoptosis assays for detecting early and overdue apoptosis or distinguishing apoptosis from necrosis and autophagy. next chapters concentrate on fresh advances in actual time and high-throughput assays that realize and quantitate apoptosis and apoptosis signaling pathways. ultimate chapters specialise in fresh advancements in preclinical anticancer therapeutics focusing on apoptosis. Written for the Methods in Pharmacology and Toxicology sequence, chapters function step by step descriptions of the methodologies, in addition to specialist tips and implementation advice.

Vital and authoritative, Apoptosis tools in Toxicology serves beginner scientists in addition to specialists, using a number of tools from universal laboratory gear to high-end dear and automatic equipment in a position to acting genuine time apoptotic measurements.

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When attempting to select an appropriate apoptosis detection method, it is essential to carefully analyze the purpose and the end goal of the experiment. Our lab is primarily interested in the detection of apoptosis in the context of therapies that target apoptosis signaling components to either induce or inhibit apoptosis in target cells. In this regard, it is our opinion that selectivity and early detection is critical. Also, it is important to select a platform that can distinguish between growth arrest and true cell death.

Although caspase activation appears to be a universal biochemical event in the apoptosis process, caspase 3/7 independent apoptosis has also been reported [4, 11]. Since every biochemical feature of apoptosis may not occur in all toxicant–cell systems, or may be transient, the use of single biochemical apoptosis endpoint assays needs to be avoided. Thus, the best approaches for quantitating apoptosis involve the utilization of a combination of assays focusing on various different biochemical and molecular features of apoptosis, with the addition of wellknown apoptosis morphological features [4, 6, 7, 11, 12].

De Graaf AO, van den Heuvel LP, Dijkman HB, de Abreu RA, Birkenkamp KU, de Witte T et al (2004) Bcl-2 prevents loss of mito- Detection of Apoptosis: From Bench Side to Clinical Practice 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. chondria in CCCP-induced apoptosis. Exp Cell Res 299:533–540 Terauchi S, Yamamoto T, Yamashita K, Kataoka M, Terada H, Shinohara Y (2005) Molecular basis of morphological changes in mitochondrial membrane accompanying induction of permeability transition, as revealed by immuno-electron microscopy.

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